6ZSM

Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	121.540, 121.540, 96.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 1.950
R-factor	0.1589
Rwork	0.157
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ya9
RMSD bond length	0.018
RMSD bond angle	2.166
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.460	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	53375	3888
<I/σ(I)>	22.8	1.9
Completeness [%]	100.0	100
Redundancy	13.3	13.8
CC(1/2)	1.000	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	292	0.20 M sodium formate, 0.1 M Bis-Tris-Propane buffer pH 8.5, 19% (w/v) PEG 3350