6ZG9
Structure of M1-StaR-T4L in complex with GSK1034702 at 2.5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.363, 66.571, 153.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.970 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y00 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.551 | 2.627 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.198 | 1.857 |
| Number of reflections | 14062 | 506 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 91.2 | |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.1 NaHEPES pH 7.4-7.8, 0.1M di-ammonium hydrogenohosphate, 30-38% PEG300 |
