6Z86
human GTP cyclohydrolase I in complex with 7-deaza-GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.999880 |
Spacegroup name | P 1 |
Unit cell lengths | 87.861, 88.595, 163.567 |
Unit cell angles | 85.22, 88.91, 83.55 |
Refinement procedure
Resolution | 162.990 - 2.206 |
R-factor | 0.1832 |
Rwork | 0.183 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fb1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.739 | 2.457 |
High resolution limit [Å] | 2.206 | 2.206 |
Rmerge | 0.045 | 0.337 |
Number of reflections | 142765 | 7139 |
<I/σ(I)> | 6.2 | 1.5 |
Completeness [%] | 89.7 | 62.4 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.1 M Morpheus Buffer 1, 30 % EDO_P8K and Morpheus ethylene glycols (Morpheus HT-96; E10; Molecular Dimensions) |