6YF2
FKBP12 in complex with the BMP potentiator compound 6 at 1.03A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99996 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 57.482, 57.482, 54.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.770 - 1.030 |
| R-factor | 0.1413 |
| Rwork | 0.141 |
| R-free | 0.14880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dh0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 1.060 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.044 | 0.140 |
| Rmeas | 0.046 | 0.166 |
| Number of reflections | 44575 | 2585 |
| <I/σ(I)> | 32.8 | 6.3 |
| Completeness [%] | 97.6 | 78.4 |
| Redundancy | 10.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.9 M AmSO4, 0.15 M CdCl2 |
