6Y73
The crystal structure of human MACROD2 in space group P43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91589 |
Spacegroup name | P 43 |
Unit cell lengths | 96.780, 96.780, 261.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.390 - 1.700 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iqy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.352 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.390 | 48.390 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.123 | 0.080 | 1.150 |
Rmeas | 0.133 | 0.085 | 1.242 |
Total number of observations | 1823516 | ||
Number of reflections | 261897 | 2973 | 19354 |
<I/σ(I)> | 8.98 | 23.02 | 1.41 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 6.963 | 7.491 | 7.063 |
CC(1/2) | 0.995 | 0.995 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.2 M Ammonium tartarate dibasic pH 6.7, 20% PEG 3350 |