6XK9

Cereblon in complex with DDB1, CC-90009, and GSPT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	P 1 2 1
Unit cell lengths	157.616, 112.041, 176.582
Unit cell angles	90.00, 95.79, 90.00

Refinement procedure

Resolution	74.450 - 3.640
R-factor	0.2097
Rwork	0.208
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hxb
RMSD bond length	0.003
RMSD bond angle	0.673
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.450	3.770
High resolution limit [Å]	3.640	3.640
Rmerge	0.118	0.471
Rmeas	0.142	0.564
Rpim	0.077	0.308
Number of reflections	68917	6809
<I/σ(I)>	4.49	2.33
Completeness [%]	99.8
Redundancy	3.3
CC(1/2)	0.992	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG 3350, 100mM Tris-HCl (pH 7.5), 300mM sodium citrate