6XAG

Apo BRAF dimer bound to 14-3-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	61.504, 115.752, 153.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.330 - 3.300
R-factor	0.2518
Rwork	0.250
R-free	0.29970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u2h
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1-3660_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.330	3.418
High resolution limit [Å]	3.300	3.300
Rmeas	0.181	0.992
Number of reflections	16106	3218
<I/σ(I)>	8.13	1.14
Completeness [%]	94.3	98.38
Redundancy	2
CC(1/2)	0.998	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	100 mM HEPES pH 7.5, 200 mM ammonium sulfate, 23% (w/v) PEG 3350