6WIH

N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	86.350, 86.350, 245.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.972 - 1.900
R-factor	0.1523
Rwork	0.151
R-free	0.18610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w1d
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.972	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	74322	7325
<I/σ(I)>	17.66
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	288	0.1 M MES pH 5.5 22.5 % PEG 400