6WDQ

IL23/IL23R/IL12Rb1 signaling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	75.712, 118.483, 186.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.990 - 3.400
R-factor	0.243
Rwork	0.238
R-free	0.29460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mzv D_1000248020
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	3.522
High resolution limit [Å]	3.400	3.400
Rmerge	0.072	2.118
Rmeas	0.089	2.602
Rpim	0.051	1.484
Number of reflections	21806	2240
<I/σ(I)>	8.59	0.57
Completeness [%]	89.7	75.95
Redundancy	2.7	2.7
CC(1/2)	0.999	0.286

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.4	295	PEG 12000 CAPSO pH 9.4 5% dioxane 10% spermine HCl