6VWU

X-ray structure of ALKS 4230, a fusion of circularly permuted human Interleukin-2 and Interleukin-2 Receptor alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-08-12
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	86.809, 86.809, 119.576
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.645 - 3.400
R-factor	0.2768
Rwork	0.275
R-free	0.29730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z92
RMSD bond length	0.002
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.645	3.522
High resolution limit [Å]	3.400	3.400
Rmerge	0.185	0.185
Number of reflections	7538	750
<I/σ(I)>	12.8
Completeness [%]	100.0	100
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	An equal volume of protein was mixed with crystallization buffer, 25 % (w/v) PEG 3350, 0.2 M MgCl2, 0.1 M Hepes 7.5. Large crystals appeared within 48-72 hours, and were frozen in a cryoprotectant consisting of mother liquor with 10 % glycerol