6VKP

Crystal structure of DPO4 extension past 8-oxoadenine (oxoA) and dG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	53.098, 103.690, 99.105
Unit cell angles	90.00, 89.94, 90.00

Refinement procedure

Resolution	49.550 - 2.540
Rwork	0.210
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
RMSD bond length	0.010
RMSD bond angle	1.239
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.530	2.530
Rpim	0.064	0.637
Number of reflections	35565	1729
<I/σ(I)>	15.68	1.2
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)		0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50 mM Tris 7.5 18% PEG 3350 100 mM magnesium acetate