6VGI
Crystal Structures of FLAP bound to MK-866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9802 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.418, 66.297, 109.430 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.610 |
| R-factor | 0.2594 |
| Rwork | 0.259 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.831 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.950 | 2.750 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 43561 | 3145 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 28% PEG 550 MME, 200 mM Ammonium Sulfate, 100mM Sodium Acetate pH 4.6, 1 mM MK-886 |
