6V0O

PRMT5 bound to the PBM peptide from pICln

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	I 2 2 2
Unit cell lengths	100.010, 136.400, 179.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.940 - 2.860
R-factor	0.213
Rwork	0.211
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gqb
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.940	2.880
High resolution limit [Å]	2.860	2.860
Rmeas	0.130	2.020
Number of reflections	28182	564
<I/σ(I)>	8.37	0.62
Completeness [%]	98.1	97.1
Redundancy	3.7	3.4
CC(1/2)	0.997	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	281	30% PEG 3350 200 mM ammonium sulfate 0.05% w/v dodecyl-maltoside