6SQS

Crystal structure of cat phospho-Ser429 MDM2 RING domain bound to UbcH5B-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.916
Spacegroup name	P 1
Unit cell lengths	54.593, 56.438, 60.742
Unit cell angles	66.44, 69.44, 89.10

Refinement procedure

Resolution	29.200 - 1.830
Rwork	0.211
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mnj
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	1.880
High resolution limit [Å]	1.830	1.830
Number of reflections	49057	3293
<I/σ(I)>	10.2
Completeness [%]	95.8
Redundancy	1.8
CC(1/2)	0.996	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.1 M Tris-HCl, pH 8.0, 15% (w/v) PEG 2000 MME and 0.1 M KCl