6SGE

Crystal structure of Human RHOB-GTP in complex with nanobody B6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.965
Spacegroup name	P 1 21 1
Unit cell lengths	66.840, 70.227, 71.212
Unit cell angles	90.00, 107.68, 90.00

Refinement procedure

Resolution	47.175 - 1.500
R-factor	0.1615
Rwork	0.161
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv8
Data reduction software	XDS (3.20)
Data scaling software	XDS (3.20)
Phasing software	PHENIX (1.16-3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.795	48.795	1.380
High resolution limit [Å]	1.300	3.900	1.300
Rmerge	0.029	0.019	1.045
Rmeas	0.035	0.024	1.459
Total number of observations	311846
Number of reflections	110991	5722	3042
<I/σ(I)>	17.39	55.2	0.61
Completeness [%]	72.5	97.2	12.3
Redundancy	2.81	3.059	1.449
CC(1/2)	0.999	0.999	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	100 mM Mes pH 6.5 200 mM MgCl2 25% v/w PEG 4000