6SF2
Ternary complex of human bone morphogenetic protein 9 (BMP9) growth factor domain, its prodomain and extracellular domain of activin receptor-like kinase 1 (ALK1).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | P 61 |
Unit cell lengths | 72.610, 72.610, 438.485 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.880 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.154 | |
Rmeas | 0.163 | |
Rpim | 0.052 | 0.369 |
Number of reflections | 19622 | 2834 |
<I/σ(I)> | 13.3 | 2.8 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 9.9 | 10.2 |
CC(1/2) | 0.997 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.14 M potassium sodium tartrate, 14% PEG 3350 |