6RPR
LEM domain of Emerin mutant T43I in complex with BAF dimer and the Igfold of the lamin A/C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-06-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.200, 81.490, 64.880 |
| Unit cell angles | 90.00, 121.37, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.260 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ghd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.740 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.096 | |
| Rmeas | 0.106 | |
| Rpim | 0.050 | |
| Number of reflections | 16448 | 16448 |
| <I/σ(I)> | 11.74 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 3350, 100 mM Tris Bis pH 5.5, 0.1 M NH4SO4 |
