6RMV

The crystal structure of a TRP channel peptide bound to a G protein beta gamma heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9754
Spacegroup name	P 21 21 21
Unit cell lengths	45.860, 80.432, 113.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.520 - 1.940
R-factor	0.1896
Rwork	0.188
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xhm
RMSD bond length	0.007
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.520	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.073	1.280
Rmeas	0.087	1.510
Rpim	0.046	0.795
Number of reflections	31659	3044
<I/σ(I)>	13.2	1.4
Completeness [%]	99.3	98.3
Redundancy	6.5	6.7
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	0.1 M HEPES sodium pH 7.5, 15% (w/v) polyethylene glycol 4,000, 7.5% (v/v) isopropanol