6QIP

Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-biniding side chain of the human growth hormone derivative somapacitan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	77.020, 114.450, 159.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.479 - 2.450
R-factor	0.2182
Rwork	0.216
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k71
RMSD bond length	0.011
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.140	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.084	1.683
Rmeas	0.091	1.831
Rpim	0.035	0.718
Number of reflections	52497	5168
<I/σ(I)>	17.08	1.12
Completeness [%]	99.9	99.9
Redundancy	6.6	6.4
CC(1/2)	0.999	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	sodium cacodylate, PEG3350