6QIO

Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	75.290, 108.120, 159.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.846 - 1.950
R-factor	0.1845
Rwork	0.182
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k71
RMSD bond length	0.012
RMSD bond angle	1.187
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	95478
<I/σ(I)>	11.23
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	ammonium acetate, sodium acetate, PEG4000