6PZV
Crystal Structure of Bovine DNMT1 RFTS domain in complex with H3K9me3 and Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.322, 196.445, 67.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 3.010 |
| R-factor | 0.2217 |
| Rwork | 0.216 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 3.118 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.126 | |
| Number of reflections | 19128 | 1774 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.1 | |
| Redundancy | 9.8 | |
| CC(1/2) | 0.998 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 277 | 0.1M citric acid (pH 3.5), 28% PEG8000 |
