6PW3
LARP1 DM15 FYRE (F844Y, R847E) mutant bound to m7GpppG dinucleotide (capG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.582, 87.355, 72.890 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 29.240 - 2.340 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zc4 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.050 | 0.580 |
| Number of reflections | 30894 | 2210 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM Hepes 7.5, 0.08 M NaCl, 36% PEG 3350 |
