6PNX
Crystal Structure of an Asymmetric Dimer of FGF Receptor 3 Kinases Trapped in A-loop Tyrosine Transphosphorylation Reaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.686, 93.253, 75.490 |
| Unit cell angles | 90.00, 103.06, 90.00 |
Refinement procedure
| Resolution | 39.378 - 2.199 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k33 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.580 | 2.330 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmeas | 0.076 | 1.030 |
| Number of reflections | 73565 | 5907 |
| <I/σ(I)> | 14 | 1.6 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M Hepes, pH 7.5, and 1.8 M (NH4)2SO4 |
