6OQX

Human Liver Receptor Homolog-1 bound to the agonist 5N and a fragment of the Tif2 coregulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	273
Detector technology	CCD
Collection date	2015-10-15
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	46.547, 46.547, 220.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.052 - 2.004
R-factor	0.2076
Rwork	0.206
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l11
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	17346	1689
<I/σ(I)>	21.3
Completeness [%]	99.9
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium acetate (pH 4.6), PEG4000, glycerol