6OB0

Compound 2 bound structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in HEK293-F cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	153.430, 191.420, 97.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.150 - 2.810
R-factor	0.2
Rwork	0.198
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oaz
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BUSTER (2.11.7)
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.100	119.720	2.820
High resolution limit [Å]	2.810	13.030	2.810
Rmerge	0.148		0.015
Rmeas	0.162		0.016
Rpim	0.064		0.610
Number of reflections	70015	793	693
<I/σ(I)>	12	33.4	2.3
Completeness [%]	99.1	97.3	99.7
Redundancy	6.4
CC(1/2)	0.998		0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.15M calcium acetate, 18% PEG3350