6NIP
Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ1 in complex with ZIKV E glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 417.330, 69.300, 212.580 |
Unit cell angles | 90.00, 112.96, 90.00 |
Refinement procedure
Resolution | 14.987 - 4.160 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mtx 5ire |
RMSD bond length | 0.004 |
RMSD bond angle | 0.680 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.250 |
High resolution limit [Å] | 4.100 | 4.100 |
Rpim | 0.190 | 0.560 |
Number of reflections | 32942 | 3339 |
<I/σ(I)> | 3.7 | 1.04 |
Completeness [%] | 75.6 | 77.5 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 0.06 M MgCl2, 0.1M Imidazole MES monohydrate (pH 6.5), 20% Ethylene glycol and 10% PEG 8,000 |