6NFM

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B with loop 7 from APOBEC3G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	50.314, 50.314, 149.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.766 - 2.530
R-factor	0.2207
Rwork	0.218
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqd
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_3357: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.620
High resolution limit [Å]	2.530	2.530
Rmerge	0.180	1.900
Rmeas	0.190	1.900
Rpim	0.070	0.700
Number of reflections	6968	674
<I/σ(I)>	10.9	1.2
Completeness [%]	99.9	100
Redundancy	7.5	7.8
CC(1/2)	0.996	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	LiCl, HEPES,PEG3350