6NFM

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B with loop 7 from APOBEC3G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA dC->dU-editing enzyme APOBEC-3B	polymer	193	22844.8	1	UniProt (Q9UH17) Pfam (PF05240) In PDB	Homo sapiens (Human)	A3B,Phorbolin-1-related protein,Phorbolin-2/3
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 187 - 378 (UniProt: Q9UH17)

PDB	External Database	Details
Met 186	-	initiating methionine
Ser 200	Phe 200	engineered mutation
Ser 228	Trp 228	engineered mutation
Lys 230	Leu 230	engineered mutation
Ser 250	Ala 242	engineered mutation
-	Lys 243	deletion
-	Asn 244	deletion
-	Leu 245	deletion
-	Leu 246	deletion
-	Cys 247	deletion
-	Gly 248	deletion
-	Phe 249	deletion
-	Tyr 250	deletion
Lys 308	Phe 308	engineered mutation
Asp 315	Tyr 315	engineered mutation
Gln 316	Asp 316	engineered mutation
Gly 317	Pro 317	engineered mutation
Arg 318	Leu 318	engineered mutation
Cys 319	Tyr 319	engineered mutation
Gln 320	Lys 320	engineered mutation
Leu 379	-	expression tag
Glu 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22844.8
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	22880.3

*Water molecules are not included.