6MPF
Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a 2-thiocytidine (s2C32) and inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 1 (TRNAARG1) bound to an mRNA with an CGC-codon in the A-site and paromomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2010-08-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 402.330, 402.330, 176.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.563 - 3.330 |
R-factor | 0.1781 |
Rwork | 0.178 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.602 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.563 | 3.449 |
High resolution limit [Å] | 3.330 | 3.330 |
Number of reflections | 375418 | 20244 |
<I/σ(I)> | 12.98 | 1.42 |
Completeness [%] | 97.7 | 97.82 |
Redundancy | 3.1 | 3 |
CC(1/2) | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K |