6MII

Crystal structure of minichromosome maintenance protein MCM/DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-26
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	186.665, 186.665, 281.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.698 - 3.150
R-factor	0.1908
Rwork	0.188
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4R7Y & 2VL6
RMSD bond length	0.007
RMSD bond angle	1.044
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.119	1.789
Rmeas	0.036	2.007
Rpim	0.125	0.877
Number of reflections	84725	7600
<I/σ(I)>	17.6	0.5
Completeness [%]	98.0	89.6
Redundancy	11.1	4.5
CC(1/2)		0.245

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	50 mM MES, pH 6.0, 100 mM sodium chloride, 20 mM magnesium chloride, 9% PEG8000