6MAJ

HBO1 is required for the maintenance of leukaemia stem cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95365
Spacegroup name	H 3
Unit cell lengths	111.543, 111.543, 73.702
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.595 - 2.139
R-factor	0.1967
Rwork	0.195
R-free	0.23740
RMSD bond length	0.004
RMSD bond angle	0.733
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.600	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.198	1.292
Rpim	0.095	0.614
Number of reflections	18887	1486
<I/σ(I)>	5.7	1.9
Completeness [%]	100.0	100
Redundancy	5.3	5.3
CC(1/2)	0.987	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Crystals were grown at 20 C in sitting drop plates in drops of 200 nL crystallant and 200 nL protein. The protein was at 2.5 mg/mL and the reservoir was 20% PEG 3350 with 244 mM diammonium tartrate.