6M11
Crystal structure of Rnase L in complex with Sunitinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2018-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.240, 111.000, 262.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.260 - 2.460 |
| R-factor | 0.2234 |
| Rwork | 0.220 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o1p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.851 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.260 | 2.548 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.023 | 0.323 |
| Number of reflections | 63535 | 63535 |
| <I/σ(I)> | 14.17 | |
| Completeness [%] | 99.3 | |
| Redundancy | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG4000, 100 mM Tris, 150 mM (NH4)2SO4 and 2 mM DTT buffer, pH 7.5 |
