6LVM
Crystal structure of FGFR3 in complex with pyrimidine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-12-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.143, 61.225, 59.645 |
| Unit cell angles | 90.00, 112.98, 90.00 |
Refinement procedure
| Resolution | 29.360 - 2.530 |
| R-factor | 0.2109 |
| Rwork | 0.207 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.580 |
| High resolution limit [Å] | 2.530 | 6.890 | 2.540 |
| Rmerge | 0.073 | 0.055 | 0.370 |
| Number of reflections | 10740 | 536 | 506 |
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 99.4 | 94 | 96.4 |
| Redundancy | 3.6 | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 296 | Citric Acid, PEG 8000 |
