6KYV

Crystal Structure of RIG-I and hairpin RNA with G-U wobble base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	112.166, 175.130, 308.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.560 - 3.000
R-factor	0.2248
Rwork	0.222
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f9f
RMSD bond length	0.001
RMSD bond angle	0.405
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.565	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.168	1.400
Rmeas	0.182	1.526
Rpim	0.070	0.594
Number of reflections	116928	5596
<I/σ(I)>	15.44	1.76
Completeness [%]	95.5
Redundancy	6.3	5.8
CC(1/2)	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	15% PEG 3350 (w/v), 100mM MES pH 6.5, 100mM NaSCN, 20mM Na Citrate tribasic