6KQQ

NSD1 SET domain in complex with BT3 and SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-25
Detector	MARRESEARCH
Wavelength(s)	0.97856
Spacegroup name	P 1
Unit cell lengths	40.469, 60.811, 64.255
Unit cell angles	101.18, 107.76, 93.58

Refinement procedure

Resolution	30.350 - 1.800
R-factor	0.1651
Rwork	0.163
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.004
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.680	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	50805	2529
<I/σ(I)>	17.42	2.36
Completeness [%]	97.3	95.4
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	18% PEG 8000, 100mM sodium cacodylate, pH 6.5, 200mM calcium Acetate