6JTZ
Crystal Structure of hRecQ1_D2-Zn-WH containing mutation on beta-hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-11-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.950, 101.835, 73.434 |
| Unit cell angles | 90.00, 99.07, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.797 |
| R-factor | 0.2293 |
| Rwork | 0.227 |
| R-free | 0.28170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1x |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.516 | 19.690 | 2.950 |
| High resolution limit [Å] | 2.797 | 8.850 | 2.800 |
| Rmerge | 0.031 | 0.366 | |
| Rmeas | 0.100 | 0.036 | 0.426 |
| Rpim | 0.051 | 0.019 | 0.217 |
| Number of reflections | 18892 | 567 | 2709 |
| <I/σ(I)> | 12.2 | 19.7 | 2.1 |
| Completeness [%] | 99.0 | 90.6 | 98 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 65% v/v Tacsimate pH=7.0 |
