6J65

Crystal structure of human HINT1 mutant complexing with AP4A II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-10
Detector	RAYONIX MX-225
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	46.300, 46.573, 64.246
Unit cell angles	98.06, 89.82, 118.25

Refinement procedure

Resolution	32.460 - 1.420
R-factor	0.181
Rwork	0.180
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eqe
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.065	0.427
Number of reflections	78869
<I/σ(I)>	20.4
Completeness [%]	89.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	HEPES pH 7.5, PEG3 350