6IWD
The PTP domain of human PTPN14 in a complex with the CR3 domain of HPV18 E7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.573, 169.153, 42.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.987 - 1.800 |
R-factor | 0.1873 |
Rwork | 0.186 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bzl |
RMSD bond length | 0.007 |
RMSD bond angle | 0.774 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.087 | 0.328 |
Number of reflections | 35719 | 1615 |
<I/σ(I)> | 41.3 | |
Completeness [%] | 95.1 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris-HCl pH 8.0, 25% (w/v) polyethylene glycol 3350, 0.25 M lithium sulfate monohydrate, and 2% (v/v) tert-butanol |