6IRZ

Crystal structure of the zebrafish cap-specific adenosine methyltransferase bound to SAH and m7G-capped RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	71.669, 83.165, 93.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.566 - 2.000
R-factor	0.1823
Rwork	0.181
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6irx
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.900	46.900	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.063	0.047	0.654
Rmeas	0.069	0.051	0.706
Rpim	0.026	0.020	0.265
Total number of observations	259727
Number of reflections	38329	508	2771
<I/σ(I)>	16.1
Completeness [%]	100.0	99.6	100
Redundancy	6.8	6.1	7.1
CC(1/2)	0.998	0.997	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	13% PEG 3350, 0.1M potassium thiocyanate, 0.1M bis-Tris propane