6I7Q
Structure of pVHL-elongin B-elongin C (VCB) in complex with hydroxylated-HIF-2alpha (523-542) in the C2221 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.827, 86.758, 136.376 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.527 - 1.798 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lqb |
RMSD bond length | 0.018 |
RMSD bond angle | 1.399 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.560 | 1.850 |
High resolution limit [Å] | 1.798 | 1.798 |
Rmerge | 0.087 | 1.042 |
Rmeas | 0.097 | 1.166 |
Rpim | 0.043 | 1.171 |
Number of reflections | 45358 | 3301 |
<I/σ(I)> | 11.9 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | 9.8 |
CC(1/2) | 0.998 | 0.643 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 0.1M Cacodyl-Na pH 5.8, 0.1M Mg formate, 50 mM Cystamine, 22 % PEG3350 |