6I1L

Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	78.800, 188.580, 284.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 2.980
R-factor	0.2066
Rwork	0.204
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nfv
RMSD bond length	0.005
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.140	3.086
High resolution limit [Å]	2.980	2.980
Number of reflections	87503
<I/σ(I)>	18.87	2.22
Completeness [%]	100.0	100
Redundancy	26.2	27.6
CC(1/2)	0.999	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.6 M trisodium citrate