6I0M

Structure of human IMP dehydrogenase, isoform 2, bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.99990
Spacegroup name	I 4 2 2
Unit cell lengths	134.729, 134.729, 324.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	162.000 - 2.567
R-factor	0.2203
Rwork	0.219
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z87
RMSD bond length	0.003
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.12rc1_2801: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	162.000	2.753
High resolution limit [Å]	2.567	2.567
Rmerge	0.194	2.617
Rmeas	0.198	2.696
Rpim	0.039	0.517
Number of reflections	48045	517
<I/σ(I)>	14.6	1.6
Completeness [%]	94.9	64.2
Redundancy	25.8	27.1
CC(1/2)	0.997	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium citrate, pH 5.5 0.2 M lithium sulphate 15% (v/v) ethanol