6HYT
Crystal structure of DHX8 helicase domain bound to ADP at 2.3 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.947, 167.576, 137.231 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 33.800 - 2.330 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 2.370 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.088 | 1.554 |
| Number of reflections | 127918 | |
| <I/σ(I)> | 8.9 | 0.9 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.998 | 0.293 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% 2-Methyl-2,4-pentanediol, 100 mM Sodium Acetate, 6%DMSO |
