6GR8
Human AURKC INCENP complex bound to BRD-7880
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.650, 72.480, 83.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.610 - 1.750 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.21626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4af3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.650 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.100 | |
| Number of reflections | 34077 | 1433 |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 97.1 | 77.3 |
| Redundancy | 5.4 | 4.1 |
| CC(1/2) | 0.995 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M sodium chloride, 25% PEG3350, 0.1 M HEPES pH 7.5 |
