6GHD
Structural analysis of the ternary complex between lamin A/C, BAF and emerin identifies an interface disrupted in autosomal recessive progeroid diseases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9725 |
Spacegroup name | P 1 |
Unit cell lengths | 57.770, 62.750, 64.340 |
Unit cell angles | 66.44, 66.55, 88.05 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.152 | 6.520 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.117 | 0.795 |
Rmeas | 0.139 | 0.958 |
Rpim | 0.074 | 0.526 |
Number of reflections | 83934 | |
<I/σ(I)> | 5.9 | |
Completeness [%] | 95.7 | |
Redundancy | 3.52 | |
CC(1/2) | 0.992 | 0.667 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 25% PEG3.350, 20mM Tris-BispH5.5, 30mM NaCl, 0.2M NH4SO4 |