6GE6

X-ray structure of TEAD4(E263A+Y429F mutant) complexed with YAP(wildtype): The role of residual flexibility and water molecules in the adaptation of a bound intrinsically disordered protein to mutations at a binding interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99993
Spacegroup name	P 41 21 2
Unit cell lengths	58.902, 58.902, 158.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.490 - 1.800
R-factor	0.2196
Rwork	0.219
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.006
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.490	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.085	0.812
Rmeas	0.088	0.845
Total number of observations	342171
Number of reflections	26774
<I/σ(I)>	23	3.8
Completeness [%]	99.9	99.9
Redundancy	12.8	13.1
CC(1/2)	0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	2M ammonium sulfate, 0.1M Bis-TRIS