6FXN

Crystal structure of human BAFF in complex with Fab fragment of anti-BAFF antibody belimumab

Replaces:  6ERX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	135.687, 135.499, 138.180
Unit cell angles	90.00, 91.88, 90.00

Refinement procedure

Resolution	138.110 - 2.900
R-factor	0.19106
Rwork	0.191
R-free	0.23128
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kd7 7fab
RMSD bond length	0.010
RMSD bond angle	1.524
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	138.110	3.150
High resolution limit [Å]	2.900	2.900
Number of reflections	109365	23126
<I/σ(I)>	11.24	3.05
Completeness [%]	98.7	95.4
Redundancy	3.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	9% (w/v) PEG4000, 0.1 M MgCl2, 0.1 M HEPES pH 7.5