6FWH
Acanthamoeba IGPD in complex with R-C348 to 1.7A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.690, 156.100, 117.120 |
| Unit cell angles | 90.00, 125.42, 90.00 |
Refinement procedure
| Resolution | 95.440 - 1.790 |
| R-factor | 0.15129 |
| Rwork | 0.149 |
| R-free | 0.19016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ekw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.512 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.440 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.076 | 0.535 |
| Rpim | 0.058 | 0.309 |
| Number of reflections | 228093 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M calcium acetate and 20 % (w/v) PEG3350 |
