6FM4
The crystal structure of S. aureus Gyrase complex with ID-130 and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 61 |
| Unit cell lengths | 92.951, 92.951, 407.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.050 - 2.700 |
| R-factor | 0.2284 |
| Rwork | 0.225 |
| R-free | 0.29990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.674 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.050 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | |
| Number of reflections | 51255 | |
| <I/σ(I)> | 30.68 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295.15 | 18% PEG 5000 MME, 0.1 M BISTRIS PH 6.5 |
