6FFY
Structure of the mouse SorCS2-NGF complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-02 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.99998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 229.755, 117.706, 90.000 |
Unit cell angles | 90.00, 111.87, 90.00 |
Refinement procedure
Resolution | 60.841 - 3.900 |
R-factor | 0.2607 |
Rwork | 0.258 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eax 4msl 1wgo 4aqo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.612 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.000 | 3.850 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.340 | |
Number of reflections | 25900 | 4621 |
<I/σ(I)> | 4.8 | 1 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6 | 6.1 |
CC(1/2) | 0.990 | 0.178 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 291 | 0.225 M MES/bis-tris pH 6.6 and 6.6 % w/v PEG 6000 |